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KMID : 0362720170550090592
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Journal of the Korean Dental Association
2017 Volume.55 No. 9 p.592 ~ p.603
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The effects of ascorbic acid on the morphology and cell prol of three-dimensional stem cell spheroids
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Lee Hyun-Jin
Yeo Seong-Il
Park Jun-Beom
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Abstract
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Purpose: The effects of various concentrations of ascorbic acid on stem cell spheroids derived from intraoral areas are not
known yet. Thus, the purpose of this study is to evaluate the effects of different concentrations of ascorbic acid on the morphology and cellular viability of stem cell spheroids derived from the gingival tissues.
Materials and Methods: Stem cells were plated onto silicon elastomer-based concave microwells and grown in the presence of ascorbic acid at concentrations ranging from 0.003% to 0.3%. The morphology of the cells was viewed under an inverted microscope at day 1, 2, 3 and 5. Qualitative live/dead assay and quantitative cellular viability using Cell Counting Kit-8 were performed on day 2 and day 5.
Results: Gingiva-derived stem cells formed spheroids irrespective of ascorbic acid concentration in silicon elastomer-based concave microwells. Increase in the diameter of spheroid were seen with higher concentrations of ascorbic acid. Higher cellular viability was seen in higher concentrations of ascorbic acid.
Conclusion: Within the experimental setting, the application of ascorbic acid on stem-cell spheroids produced an increase in the size and higher viability with higher dosage. It can be suggested ascorbic acid be applied with stem cell spheroids for tissue engineering purposes.
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KEYWORD
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ascorbic acid, cell proliferation, cell survival, cellular spheroids, gingiva, stem cells
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